Journal: iScience

Article Title: circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization

doi: 10.1016/j.isci.2023.107325

Figure Lengend Snippet: eIF4A3 regulated the expression of circIFNGR2 (A) The binding sites of eIF4A3 in the flanking sequences of the IFNGR2 pre-mRNA transcript were predicted by circInteractome. (B) RIP assays for detection of the binding relationships among eIF4A3 and IFNGR2 pre-mRNA transcripts. (C) Western Blot analysis for identification of the binding relationships among eIF4A3 and the flanking sequences of IFNGR2 pre-mRNA transcripts. (D) qRT-PCR analysis of the expression level of eIF4A3 mRNA in THP-1 cells, M0 macrophages, M1 macrophages, and M2 macrophages. (E) qRT-PCR analysis of the expression level of eIF4A3 mRNA, circIFNGR2, and linear IFNGR2 with or without overexpression and knockdown of eIF4A3. (F) qRT-PCR analysis of the expression of circIFNGR2 in PBMCs from 24 healthy controls and 33 patients with AS. (G) Correlation analysis of the expression levels of circIFNGR2 and eIF4A3 mRNA in PBMCs from patients with AS. Data in B, D, and E are presented as mean ± SD, data in F are presented as median and quartiles. ∗∗: p < 0.01; ∗∗∗: p ＜0.001; ns: p ≥ 0.05.

Article Snippet: Rabbit polyclonal anti-eIF4A3 , Bioss , Cat# bs-14548R.

Techniques: Expressing, Binding Assay, Western Blot, Quantitative RT-PCR, Over Expression, Knockdown

Journal: iScience

Article Title: circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization

doi: 10.1016/j.isci.2023.107325

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-eIF4A3 , Bioss , Cat# bs-14548R.

Techniques: Virus, Recombinant, Adjuvant, In Vivo, SYBR Green Assay, Transfection, Lysis, Blocking Assay, Western Blot, DNA Extraction, Protein Extraction, Enzyme-linked Immunosorbent Assay, Purification, RNA Immunoprecipitation, Luciferase, Software

Journal: RNA

Article Title: The RNA-binding profile of Acinus, a peripheral component of the exon junction complex, reveals its role in splicing regulation

doi: 10.1261/rna.057158.116

Figure Lengend Snippet: Proportion of Acinus peaks associated with eIF4A3 peaks and enrichment at “canonical” EJC occupancy site (24-nt upstream of an exon junction). ( A ) Proportion of Acinus peaks found in close proximity of eIF4A3 peaks (eIF4A3+) (maximum distance of 10 nt between both peak summits). The analysis was done on “genomic” peaks (reads mapped to the genome) depending on their location: all peaks ( n = 36,271), exonic peaks ( n = 25,347), 5′UTR peaks ( n = 1347), 3′UTR peaks ( n = 2379), and intronic peaks ( n = 6944), but also on “transcriptomic” peaks (reads mapped to a representative transcriptome data set) ( n = 13,231). eIF4A3 CLIP-seq data described in were downloaded from GEO: GSM1001331. ( B ) Distribution of the peak frequency according to the distance of the peak summit from the exon 5′ end or the exon 3′ end. This analysis was done using Acinus’ “common transcriptomic” peak data for all peaks ( n = 10,735), peaks associated with eIF4A3 ( n = 2000), or peaks not associated with eIF4A3 ( n = 8735) (from left to right ). Only internal exons were used. The iCLIP data are represented by a red line. The gray line corresponds to randomized peak summit positions.

Article Snippet: The following commercial antibodies were used: anti-Acinus antibody (Calbiochem, ab-2 PC552), anti-T7 mouse monoclonal (Novagen, 69522), anti-eIF4A3 antibody (Proteintech, 17504-1-AP), and anti-β-tubulin (Sigma, T4026).

Techniques:

Journal: Biology of Reproduction

Article Title: Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice †

doi: 10.1093/biolre/iox150

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: 1:100 n.a Thermo EIF4E Mo Thermo MA1–089 AB_2536738 1:100 1:2000 EIF4E2 Mo Santa Cruz sc-100731 AB_1122491 1:100 n/a EIF4E3 Rab ProteinTech 17282–1-AP AB_2262162 1:100 n/a EIF4A1 Mo Thermo MA5–14901 AB_11006110 1:100 n/a EIF4A2 Rab ProteinTech 11280–1-AP AB_2097378 1:100 1:2000 EIF4A3 Rab Abcam Ab32485 AB_732124 1:50 n/a SYCP3 Rat Handel Lab. Eaker et al.,2001 n.a.

Techniques: